Product Description
TB-500 is a synthetic peptide derived from the active region of thymosin beta-4 (T-beta-4), a naturally occurring 43-amino acid protein found in nearly all mammalian cell types. Thymosin beta-4 was originally identified in the thymus gland, and it functions as the primary intracellular regulator of actin dynamics, the cytoskeletal system cells rely on for movement, shape change, and migration toward sites of injury.
The defining mechanism behind TB-500’s research relevance is its role as a G-actin sequestering protein. By binding monomeric G-actin and holding it in a readily available reserve, TB-500 increases the pool of actin that cells can rapidly polymerise into filamentous structures when migration is required, such as during wound repair. This single mechanism has downstream effects across multiple tissue repair processes, including fibroblast migration, endothelial cell sprouting, and progenitor cell mobilisation.
Pepta Supply provides TB-500 as a lyophilized powder in 10 mg vials, independently HPLC-tested and batch-verified before dispatch.
Spécifications du produit
Compound Name TB-500 (Thymosin Beta-4 fragment) Parent Protein Thymosin Beta-4 (43 amino acids) Forme Lyophilized powder Net Content 10 mg per vial Pureté 99% or above (HPLC-verified) Masse moléculaire 4,963.4 g/mol Molecular Formula C212H350N56O78S Numéro CAS 885340-08-9 Primary Mechanism G-actin sequestration, angiogenesis, cell migration Vérification Third-party HPLC, Certificate of Analysis via QR batch system Storage 2 to 8 degrees Celsius, protected from light and moisture Intended Use Research use only. Not for human or veterinary use.
Research Background
TB-500’s research base spans several decades of work on its parent molecule, thymosin beta-4, with research extending across wound healing, cardiovascular repair, musculoskeletal injury, and neurological models. Its mechanism is well characterised at the molecular level, even where translation to confirmed clinical applications remains in earlier stages.
Actin Sequestration: The Core Mechanism
Thymosin beta-4 binds monomeric G-actin with 1:1 stoichiometry and prevents its polymerisation into F-actin filaments, a finding established in landmark biochemical work confirming it as the major G-actin sequestering protein in mammalian cells. This creates a dynamic, rapidly mobilisable actin reserve. When tissue is injured, cells at the wound margin must quickly reorganise their actin cytoskeleton to migrate toward the site and begin repair, and G-actin availability is the rate-limiting step in this process. By expanding the available actin pool, TB-500 supports faster, more coordinated cell migration across multiple cell types involved in tissue repair, including fibroblasts, endothelial cells, and keratinocytes.
Angiogenesis via VEGF and MMP-2
Independent of its actin-binding function, TB-500 has been shown to upregulate vascular endothelial growth factor (VEGF) expression, with research recording increases of 2.5 to 3.8-fold in VEGF mRNA across various cell types. It also promotes angiogenesis through upregulation of matrix metalloproteinase-2 (MMP-2), an enzyme that creates controlled pathways through the extracellular matrix for new blood vessel growth. This VEGF and MMP-2-driven mechanism is mechanistically distinct from BPC-157’s primarily VEGFR2-mediated angiogenic pathway, which is why the two peptides are frequently described as complementary rather than redundant in tissue repair research, and why Pepta Supply offers them together in the BPC-157 plus TB-500 formulation.
Anti-Inflammatory and Cell Survival Effects
Research has documented that thymosin beta-4 administration reduces the inflammatory phase of injury response without suppressing the subsequent proliferative repair phase, a distinction that is mechanistically significant because excessive or prolonged inflammation can itself impair healing outcomes. TB-500 has also been studied for effects on cell survival and stem and progenitor cell mobilisation at injury sites, broadening its research relevance beyond direct structural repair to the cellular signalling environment surrounding an injury.
Tissue-Specific Research Areas
Preclinical research has investigated TB-500 across a range of tissue types. In cardiovascular models, the focus has been on post-myocardial infarction repair, where its angiogenic and cell survival properties are studied for their role in promoting collateral circulation and cardiomyocyte survival following ischemic injury. In neurological models, thymosin beta-4-related research has explored neuroprotective effects in the context of stroke and traumatic brain injury, centred on neuronal survival and regeneration pathways. Musculoskeletal research, covering muscle, tendon, ligament, and cartilage, remains the most extensively studied tissue category, building on the actin-sequestration and angiogenesis mechanisms described above.
Note: Most available data on TB-500 comes from preclinical and animal models. Human clinical evidence for the full thymosin beta-4 molecule exists under separate development programs (RGN-259 for dry eye, RGN-137 for chronic wounds), which share the active region but are not identical compounds to TB-500. No large-scale human trials of TB-500 specifically have been published, and it has not received regulatory approval for any therapeutic indication.
Batch Verification and Quality
Each vial of TB-500 from Pepta Supply carries a unique batch reference number. Independent third-party HPLC testing is carried out on every batch before it ships, confirming compound identity and purity at or above 99%. The resulting Certificate of Analysis is accessible via the QR code on the vial or through peptasupply.com/v/.
Storage and Handling
Store lyophilized powder at 2 to 8 degrees Celsius, protected from light and moisture. Reconstitute with bacteriostatic water following standard laboratory protocols. Do not freeze the reconstituted solution. Use standard aseptic technique throughout handling and preparation. Consult relevant laboratory protocols for synthetic peptide handling appropriate to the research application.
Research Use Only. TB-500 is supplied exclusively for scientific and laboratory research. Not intended for human consumption, therapeutic, diagnostic, clinical, or veterinary use. Pepta Supply B.V. accepts no liability for misuse or non-compliant use. Buyers are fully responsible for compliance with applicable regulations in their country.
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About the Compound
What is TB-500?
TB-500 is a synthetic peptide derived from the active region of thymosin beta-4, a naturally occurring 43-amino acid protein found in nearly all mammalian cells. It is studied primarily for its role as a G-actin sequestering protein, which underlies its effects on cell migration, tissue repair, and angiogenesis.
What does G-actin sequestration mean?
Actin exists in two forms inside cells: monomeric G-actin and filamentous F-actin, which is built from polymerised G-actin subunits. TB-500 binds G-actin in a 1:1 ratio and prevents it from polymerising, creating a readily available reserve. When cells need to migrate, such as during wound repair, they rapidly draw on this reserve to build the actin filaments required for movement. G-actin availability is the rate-limiting step in this process, which is why expanding the available pool accelerates cell migration.
How does TB-500 promote angiogenesis?
Independent of actin sequestration, TB-500 has been shown to upregulate VEGF expression, with research recording 2.5 to 3.8-fold increases in VEGF mRNA across various cell types. It also promotes angiogenesis through upregulation of MMP-2, an enzyme that creates pathways through the extracellular matrix for new vessel growth. This combined mechanism is distinct from BPC-157's primarily VEGFR2-mediated pathway.
What is the relationship between TB-500 and BPC-157?
Both are studied for their roles in tissue repair and angiogenesis, but through different mechanisms. TB-500 works primarily through actin sequestration and VEGF or MMP-2-mediated angiogenesis, contributing at the cellular migration level. BPC-157 works through VEGFR2-Akt-eNOS signalling, contributing at the vascular remodelling and growth factor level. Because these mechanisms are considered complementary rather than redundant, the two are frequently studied together and are available from Pepta Supply both individually and as the BPC-157 plus TB-500 combined formulation.
Is TB-500 the same as thymosin beta-4?
TB-500 is derived from the active region of thymosin beta-4 and retains many of its biological activities, but it is a distinct synthetic peptide rather than the identical full-length molecule. Clinical development programs for the full thymosin beta-4 molecule exist under different names (RGN-259 for dry eye, RGN-137 for chronic wounds) and share the active region with TB-500, but are not the same compound.
What tissue types has TB-500 been studied in?
Preclinical research has covered musculoskeletal tissue (muscle, tendon, ligament, cartilage), which remains the most extensively studied category, as well as cardiovascular models investigating post-myocardial infarction repair and collateral circulation, and neurological models exploring neuroprotective effects following stroke or traumatic brain injury.
Quality and Testing
What purity does Pepta Supply's TB-500 meet?
Pepta Supply's TB-500 is tested to a purity of 99% or above, confirmed by independent third-party HPLC analysis on each batch. The test data is produced externally and linked directly to the batch identifier on the vial.
Where can I access the Certificate of Analysis?
Scan the QR code on the vial, or visit peptasupply.com/v/ and enter the batch number. The batch-specific Certificate of Analysis and HPLC data will be displayed. This documentation is publicly accessible and does not require a login or completed purchase.
Ordering and Shipping
What size does TB-500 come in?
TB-500 from Pepta Supply is available as a 10 mg vial, supplied as a lyophilized powder. It is sold individually and is also available as part of the BPC-157 plus TB-500 compound formulation and the Glow Blend multi-compound vial.
What is the delivery timeline for EU orders?
Orders placed before 3:00 PM on working days are dispatched the same day. Most EU destinations receive their order within 1 to 3 business days. Free shipping applies to orders over 150 euros.
How should TB-500 be stored after delivery?
Transfer the vial to refrigerated storage at 2 to 8 degrees Celsius upon receipt. Keep it away from direct light and moisture. Once reconstituted for research use, do not freeze the resulting solution.
Compliance
Is TB-500 approved for human use?
No. TB-500 has not received regulatory approval for any human therapeutic use in any jurisdiction. Pepta Supply supplies it exclusively for scientific and laboratory research by qualified professionals in controlled environments.
Who can purchase TB-500 from Pepta Supply?
TB-500 is available to qualified researchers and professionals in laboratory settings. By completing a purchase, buyers confirm they are acquiring the compound for legitimate research purposes and accept full responsibility for ensuring their use complies with all applicable laws and regulations in their country.
Content prepared for research-use-only positioning on peptasupply.com. No statements constitute medical claims or endorsement for human use. Prepared by SERPISTAN for Pepta Supply B.V.