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Where These Numbers Come From
Community TB-4 doses are conservatively extrapolated from a large body of animal research, with some reference to human safety data.
Animal Study Doses: Nearly all TB-4 research uses 6 mcg/mouse IP (~0.24 mg/kg). Using standard allometric scaling to a 70 kg human gives a human equivalent of ~17 mg single dose, or ~5-10 mg/week. Community doses (5-10 mg/week loading, 2-5 mg/week maintenance) sit in the conservative middle of this range.
Phase I Human Safety (Ruff et al., 2010): Massive IV doses of 42, 140, 420, and 1,260 mg were well-tolerated in healthy subjects — no dose-limiting toxicity. This was safety testing, not therapeutic dosing.
Key mechanisms: Actin sequestration for cell migration (Mannherz & Huff, 2011), VEGF-driven angiogenesis (Philp et al., 2003), NF-kB suppression for anti-inflammatory effects (Sosne et al., 2007), and Akt survival signaling (Bock-Marquette et al., 2004).
Stacking Protocols
TB-4 + BPC-157 (Popular Healing Stack)
TB-4
750 mcg daily (loading) / 500 mcg 2x/week (maintenance)
SC
AM
Actin remodeling, cell migration, anti-inflammatory
BPC-157
250-500 mcg daily
SC (near injury)
Angiogenesis, growth factors, GI protection
TB-4 + GHK-Cu
Per protocol
Cell migration, tissue repair
GHK-Cu
1-3 mg 2x/week
Collagen synthesis, wound remodeling
TB-4 vs TB-500
Structure
Full 43-amino-acid peptide
Synthetic 17-23 AA fragment
Weekly dose
5-10 mg (loading)
3.5 mg (500 mcg daily)
Contains LKKTET
Yes (angiogenesis domain)
No
Primary use
Broad tissue repair + angiogenesis
Focused actin migration
For the complete comparison, see TB-4 vs TB-500.
Side Effects & Safety
Injection site irritation — mild, transient
Rare mild fatigue — occasional community report
No hormonal disruption — unlike some peptides
Phase I human safety — IV doses up to 1,260 mg well-tolerated with no serious adverse events
No mutagenic or carcinogenic effects in long-term animal studies
Theoretical angiogenic concern — pro-angiogenic effects raise questions about existing tumors, though no evidence of tumor promotion exists
No long-term human data at community doses
mg to Units Conversion
On a standard 100-unit insulin syringe, each "unit" equals 0.01 mL (so 100 units = 1 mL). Once thymosin beta-4 is reconstituted, the conversion from a target dose to syringe units depends on the chosen dilution.
The two reconstitution ratios most often described in community protocols are below.
Reconstitution A: 10 mg vial + 2 mL BAC water (5 mg/mL) — the standard dilution from the Quick Reference above.
400 mcg
0.08 mL
8 units
750 mcg
0.15 mL
15 units
1100 mcg
0.22 mL
22 units
1500 mcg
0.3 mL
30 units
Reconstitution B: 10 mg vial + 3 mL BAC water (3.33 mg/mL) — more BAC water for larger, easier-to-measure draws.
0.12 mL
12 units
0.225 mL
22.5 units
0.33 mL
33 units
0.45 mL
45 units
These conversions reflect the dilutions documented in community reconstitution protocols. They report how the math is described, not a recommended dosing schedule.
Core Supplies for This Protocol
The three essentials for running any reconstituted injectable: cold storage, accurate syringes, and metabolic tracking.
Cooluli Classic 4L Mini Fridge
Compact thermoelectric mini-fridge with heat/cool toggle. The most-mentioned dedicated peptide-storage fridge in research community sources — fits ~20 vials and runs quietly.
BD Ultra-Fine 31G 0.3cc 5/16" Insulin Syringes (Box of 90)
0.3cc 31G syringes — each gradation marks 1 unit (vs 2 units on 1cc), making sub-50-unit peptide doses accurate. BD Ultra-Fine is the most widely-cited brand in peptide community sources.
CONTOUR NEXT GEN Glucose Meter All-In-One Kit
Ascensia's CONTOUR NEXT GEN — the most clinically-validated home glucose meter. Includes 20 test strips + lancing device. Tracks the blood-sugar response GLP-1 users care about, especially during dose escalation.
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