CJC-1295 (without DAC) (Modified GRF 1-29)
Short-Acting Growth Hormone Releasing Hormone Analog
Community Research
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Synthetic growth hormone releasing hormone analog with short half-life enabling pulsatile GH secretion patterns resembling natural physiology. Unlike CJC-1295 with DAC, this version preserves natural GH pulsatility without continuous elevation.
Binds GHRH receptors on somatotroph cells in the pituitary gland, stimulating cAMP production and physiological GH pulses. The brief 30-minute half-life allows pulsatile release versus prolonged elevation.
Molecular Data
Tyrosine
Position 1
Alanine
Position 2
Aspartic Acid
Position 3
Position 4
Isoleucine
Position 5
Phenylalanine
Position 6
Threonine
Position 7
Asparagine
Position 8
Serine
Position 9
Position 10
Arginine
Position 11
Lysine
Position 12
Valine
Position 13
Leucine
Position 14
Position 15
Glutamine
Position 16
Position 17
Position 18
Position 19
Position 20
Position 21
Position 22
Position 23
Position 24
Position 25
Position 26
Position 27
Position 28
Position 29
Position 30
Research Indications
Restores natural GH pulsatility patterns in aging individuals.
Elevates IGF-1 through enhanced GH secretion.
Supports pituitary axis without suppression of natural function.
Supports maintenance of lean muscle mass through GH elevation.
Supports bone health through GH-mediated pathways.
Improvements in skin quality through collagen synthesis.
Enhanced recovery from training through GH elevation.
Improved sleep architecture with bedtime dosing.
Dosing Protocols
Subcutaneous injection is the only effective route. Best administered on empty stomach, 30+ minutes away from food.
Anti-Aging/Wellness
100mcg
2x daily (morning and bedtime)
SubQ
Body Composition
100-150mcg
3x daily (morning, post-workout, bedtime)
Maximum GH Release
200mcg
2-3x daily with GHRP
Sleep Enhancement
100-200mcg
Once at bedtime
Reconstitution Instructions
Bacteriostatic water
Insulin syringes (29-31 gauge)
Alcohol swabs
Sterile work surface
1 Clean vial tops with alcohol pad and allow to dry
2 Draw bacteriostatic water slowly
3 Inject water along vial side
4 Gently roll to mix—avoid vigorous shaking
5 Verify solution clarity; cloudiness indicates degradation
6 Label with date and concentration
7 Refrigerate immediately, use within 30 days
Interactions
What to Expect
Side Effects & Safety
Common Side Effects
Generally well-tolerated at recommended doses
Temporary facial flushing/warmth (5-10 minutes post-injection)
Stop Signs - Discontinue if:
Persistent joint pain or carpal tunnel symptoms
Significant water retention or edema
Unexplained headaches or vision changes
Extreme fatigue or lethargy
Allergic reaction signs at injection sites
Contraindications
Active cancer (due to growth-promoting effects)
Diabetic retinopathy
Severe kidney disease
Pregnancy or breastfeeding
Quality Checklist
Good Signs
Vendor provides certificates of analysis (>98% purity)
Proper cold-chain shipping with ice packs/refrigeration
Vacuum-sealed vials showing pressure seal integrity
White/off-white lyophilized powder
Warning Signs
Verify expiration dates; lyophilized peptides stable ~2 years if stored properly
Bad Signs
Pre-reconstituted solutions—peptide degrades rapidly
Discolored powder (yellow/brown indicates degradation)
Cloudy solution after reconstitution
Frequently Asked Questions
How many times per day should CJC-1295 (without DAC) be injected?
CJC-1295 without DAC typically requires 2-3 injections daily (morning, post-workout optional, and bedtime) with 100-300mcg per dose to maintain GH elevation. The 30-minute to 2-hour half-life necessitates multiple daily injections, unlike the weekly DAC variant.
What time of day is best to inject CJC-1295 without DAC?
Optimal timing is upon waking on an empty stomach and before bedtime, with an optional post-workout injection. Inject 30+ minutes away from food to maximize GH release. Bedtime dosing is particularly effective as it amplifies natural sleep-associated GH pulses.
Does CJC-1295 without DAC maintain natural GH pulsatility unlike the DAC version?
Yes, the non-DAC version preserves natural pulsatile GH secretion patterns with its 30-minute half-life, whereas DAC provides continuous elevation. This pulsatility is closer to physiology and results in fewer side effects like joint pain compared to continuous DAC stimulation, making it popular for anti-aging protocols.
Can CJC-1295 be stacked with GHRP peptides for better results?
Yes, combining CJC-1295 without DAC with GHRP-6 or GHRP-2 creates a synergistic 'stack' where the GHRH and GHRP work through complementary pathways to amplify GH release significantly beyond either peptide alone. This is the classic approach for maximizing growth hormone stimulation.
References
Identified CJC-1295 as a tetrasubstituted hGRF(1-29) analog that covalently binds albumin. Showed 4-fold increase in GH area under the curve compared to native hGRF(1-29) in rat pituitary cells.
Single injection of CJC-1295 produced dose-dependent 2- to 10-fold increases in GH for 6+ days and 1.5- to 3-fold increases in IGF-I for 9-11 days in healthy adults. Estimated half-life of 5.8-8.1 days.
GH secretion was increased after CJC-1295 with preserved pulsatility. Basal (trough) GH levels were markedly increased (7.5-fold), demonstrating that CJC-1295 maintains natural GH pulse patterns.
Once-daily CJC-1295 maintained normal body composition and growth in GHRH knockout mice, demonstrating efficacy in restoring GH axis function in GH-deficient animal models.
CJC-1295 administration in normal adults activated the GH/IGF-1 axis and produced measurable serum protein profile changes, providing biomarker evidence for sustained GHRH analog activity.
Related Peptides
Complementary GH release via different receptor pathways. Popular combination.
Amplifies GH pulse when combined.
Potent combination maximizing GH through dual pathways.
Different mechanisms; continuous ghrelin versus pulsatile GHRH.
Disclaimer
This information is for educational and research purposes only. Consult a healthcare professional before use.