Thymosin Beta-4 Dosage Guide, Benefits & Side Effects
Thymosin Beta-4 speeds wound closure and angiogenesis while limiting cardiac infarct size, with Phase 3 data on corneal repair too.
Chemical Makeup
37
Amino Acids
Thymosin Beta-4
Lys
Position 1
Trp
Position 2
Position 3
Leu
Position 4
Phe
Position 5
Position 6
Position 7
Ile
Position 8
Glu
Position 9
Position 10
Val
Position 11
Gly
Position 12
Gln
Position 13
Asn
Position 14
Position 15
Arg
Position 16
Asp
Position 17
Position 18
Position 19
Position 20
Position 21
Ala
Position 22
Position 23
Pro
Position 24
Position 25
Position 26
Position 27
Position 28
Position 29
Position 30
Position 31
Position 32
Thr
Position 33
Position 34
Position 35
Position 36
Position 37
As seen on
Thymosin Beta-4 Dosage Guide for Recovery & RepairThymosin Beta-4 Dosage Calculator
Thymosin Beta-4 (also called TB-500) is a peptide that helps the body heal tissue faster, calm inflammation, and repair muscles, tendons, ligaments, and skin. It's popular for recovering from injuries and wounds.
Vial size
5 mg
Bacteriostatic water
2 mL
Dosing
1–2.5 mg
Frequency
2×/week
Cycle
4–6 weeks
Benefit
Recovery & Repair
What is Thymosin Beta-4?
Thymosin Beta-4 is a naturally occurring peptide found throughout the body that plays a central role in how cells move and rebuild themselves. It supports wound healing, helps form new blood vessels, and can mobilize stem cells to injury sites. It has reached late-stage human trials for a serious eye condition and earlier trials for skin wounds and heart repair, and it's also widely used in research and biohacking for injury recovery and reducing inflammation.
Healing
Research targets include tissue repair, wound healing, and recovery from injury.
Longevity
Research targets include cellular aging, senescence, and long-term healthspan.
Immunity
Research targets include immune cell activity, inflammatory response, and infection defense.
thymos
Is Thymosin Beta-4 FDA approved?
Thymosin Beta-4 is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.
Research Use Only
Thymosin Beta-4 has not been evaluated by the FDA for safety or efficacy in humans.
No Clinical Oversight
Not manufactured under FDA-regulated quality or clinical standards.
Unregulated Sourcing
Purity, dosing, and sourcing are not verified through FDA testing or oversight.
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How Does Thymosin Beta-4 Work?
Inside cells, it helps manage the internal scaffolding protein actin that lets cells move; outside cells, it acts more like a hormone, switching on a pathway that drives cell migration, growth, and survival. This combination helps mobilize the body's own repair cells, encourage new blood vessel growth, and limit the kind of scarring that can follow an injury.
Manages cell scaffolding
Helps manage the internal actin scaffolding that lets cells move.
Acts like a hormone
Outside cells, switches on a pathway that drives migration, growth, and survival.
Limits scarring
Mobilizes repair cells, encourages new vessels, and limits scarring.
Thymosin Beta-4 Targeted areas
How to reconstitute Thymosin Beta-4
The materials you'll need and step-by-step instructions for safely mixing Thymosin Beta-4 with bacteriostatic water.
Materials needed
Your Thymosin Beta-4 vial (lyophilized)
Alcohol swabs
Bacteriostatic sterile water
3 mL syringes (Luer Lock tip)
25G or 27G needles (Luer Lock). Other gauges may also be acceptable.
Sharps container (optional)
Remove the caps
Sanitize the rubber stoppers
Attach the needle
Draw the bac water
Pull back on the plunger to draw your desired volume of bacteriostatic water. If you overfill, just push the excess back in until you reach the right marker on the syringe.
Insert the needle into the Thymosin Beta-4 vial
With the bac water in your syringe, insert the needle into the Thymosin Beta-4 vial at a slight angle to avoid pressure buildup.
Release the water gently
Let the water run gently down the side of the vial. Don't inject it forcefully.
Swirl to dissolve
Avoid shaking. Gently swirl, flip, and roll the vial to dissolve the powder.
Check for full dissolution
Cap, dispose, and store
How to Store Thymosin Beta-4
Freeze the dry powder, keeping it dry; it can handle room temperature for a few weeks. Once mixed with bacteriostatic water, refrigerate and use within about a month, avoiding repeated freezing and thawing.
Lyophilized Storage
-20°C, desiccated; stable at room temperature for up to 3 weeks.
Reconstituted Storage
With bacteriostatic water, 2–8°C for up to 28 days.
Handling Notes
Avoid freeze-thaw cycles.
What Are the Benefits of Thymosin Beta-4?
What research says it may help with, and how it works in the body.
tissue repair
It helps tissue regenerate better and shortens healing time for surgical wounds.
It speeds up recovery from injuries to muscles, tendons, and ligaments.
In clinical trials for pressure sores (bedsores), 67% of wounds fully healed compared to only 25% with a placebo.
cardiovascular
In mid-stage clinical trials, giving it within 6 hours of a heart attack reduced the amount of damaged heart tissue by 43%.
neuroprotection
In animal studies, it improved brain-related outcomes and helped restore normal function after injury.
What Are the Side Effects of Thymosin Beta-4?
Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.
Who Should Avoid It
Active chemotherapy treatment
Severe systemic allergies to peptides
Stop Right Away If You Notice
Severe injection site reactions or persistent inflammation
Signs of systemic allergic reaction (rash, breathing difficulty)
Unexpected cardiovascular symptoms during treatment
Any signs of infection at injection sites
Milder Signs to Watch For
How Long Should a Thymosin Beta-4 Cycle Last?
This breaks down how long a typical Thymosin Beta-4 cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.
That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.
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Cheat Sheets Featuring Thymosin Beta-4
See how Thymosin Beta-4 fits into a broader research stack with these free downloadable cheat sheets. Each one includes reference dose ranges, dosing frequency, and half-life for every peptide it covers. For research purposes only.
18 peptides
15 peptides
42 peptides
78 peptides
Thymosin Beta-4 Research References
It is a phase 3 compound
Thymosin Beta-4 is a phase 3 compound
72 subjects showing significant positive effects on ocular discomfort and corneal staining with excellent safety profile.
2015
First human study in heart attack patients demonstrating potential clinical benefits in repairing damaged tissue.
2016
Complete clearing of persistent corneal defects in most subjects using topical formulation.
2010
First-in-human study establishing safety and pharmacokinetics in healthy volunteers with no dose-limiting toxicity.
Frequently Asked Questions About Thymosin Beta-4
Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringe units to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
Are peptides safe?
There is no single answer because every peptide is entirely different. Safety depends on the specific compound, how pure it is, the dose you take, the way it was made, and your own personal medical history.
Are all peptides medicines?
No, they are not all medicines. The word "peptide" just describes the shape and chemical makeup of the molecule, rather than its legal or medical status. While some are officially approved prescription drugs, many others are strictly for research or are used as standard health supplements.
What does peptide reconstitution mean?
Reconstitution is the simple process of mixing a freeze-dried powder with a safe liquid to turn it into a usable liquid solution. Whenever this is done, it requires very clean and sterile methods to prevent any harmful bacteria from getting into the mixture.
Which peptide is best for my goals?
There is rarely one single best option for everyone. Instead of looking for a magic compound, it is much safer and more effective to start by looking at your specific goals. We recommend exploring our category pages first to learn about the different options that align with what you want to achieve.