CJC-1295 DAC Reconstitution Guide: Laboratory Preparation Protocol
CJC-1295 DAC Reconstitution Guide: Laboratory Preparation Protocol
CJC-1295 with Drug Affinity Complex (DAC) represents a synthetic analog of growth hormone-releasing hormone engineered for extended biological half-life through albumin binding. This modified peptide sequence incorporates a lysine residue that facilitates covalent attachment to serum albumin, significantly prolonging its stability in aqueous solutions. Research applications focus on investigating growth hormone release mechanisms, metabolic pathway studies, and cellular signaling cascades in controlled laboratory environments.
Required Materials and Equipment
CJC-1295 DAC lyophilized vial (2mg)
Bacteriostatic water for injection or sterile water for injection
Sterile syringes (1mL and 3mL capacity)
Sterile needles (25-27 gauge)
70% isopropyl alcohol swabs
Sterile vial caps or parafilm
Laboratory balance (analytical grade)
Refrigerated centrifuge
Laminar flow hood or biosafety cabinet
Personal protective equipment (gloves, safety glasses)
Pre-Reconstitution Laboratory Preparation
Establish sterile working conditions within a laminar flow hood to prevent contamination during reconstitution procedures. Allow the lyophilized CJC-1295 DAC vial to equilibrate to room temperature for approximately 15 minutes before opening. Inspect the vial contents visually to confirm the presence of white to off-white powder with no visible discoloration or foreign particles. Prepare all sterile equipment within the aseptic environment and organize materials to minimize cross-contamination risks.
Calculate the desired final concentration based on research requirements and experimental design parameters. Consider the peptide's molecular weight of approximately 3647.28 Da when determining appropriate dilution ratios. Prepare solvent selection based on intended storage duration, with bacteriostatic water preferred for extended storage periods due to antimicrobial preservatives.
Reconstitution Procedure
Step 1: Remove the aluminum cap from the CJC-1295 DAC vial and disinfect the rubber stopper thoroughly using 70% isopropyl alcohol. Allow the alcohol to evaporate completely before proceeding to prevent interference with peptide stability.
Step 2: Draw the calculated volume of bacteriostatic water into a sterile syringe, ensuring no air bubbles remain in the solution. The volume selection depends on the desired final concentration for your specific research applications.
Step 3: Insert the needle through the rubber stopper at a 45-degree angle to minimize coring. Direct the needle tip toward the inner wall of the vial rather than directly onto the lyophilized powder to prevent foaming and protein denaturation.
Step 4: Inject the solvent slowly along the vial wall, allowing the liquid to flow gently down the sides. Avoid creating turbulence or directing the stream forcefully onto the powder, as mechanical agitation can damage the peptide structure.
Step 5: Remove the needle and gently swirl the vial in circular motions to facilitate dissolution. Avoid vigorous shaking or vortexing, which may cause protein aggregation and reduce bioactivity in subsequent research applications.
Step 6: Allow the solution to stand for 2-3 minutes to ensure complete dissolution of the lyophilized material. The resulting solution should appear clear and colorless without visible particulates or cloudiness.
Concentration Options
1.0 mL
2.0 mg/mL
High-concentration studies
2.0 mL
1.0 mg/mL
Standard research protocols
4.0 mL
0.5 mg/mL
Dilution series preparation
Storage and Stability Conditions
Store reconstituted CJC-1295 DAC solutions at 2-8°C in a laboratory refrigerator to maintain peptide integrity. Protect from direct light exposure by wrapping vials in aluminum foil or storing in opaque containers. Reconstituted solutions typically maintain stability for 2-4 weeks when stored properly with bacteriostatic water, while sterile water preparations should be used within 72 hours of reconstitution.
For extended storage requirements, prepare aliquots in sterile microcentrifuge tubes and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles, which can compromise peptide structure and biological activity. Label all containers with reconstitution date, concentration, and storage conditions for proper laboratory inventory management.
Research Application Notes
Element SARMs provides CJC-1295 DAC exclusively for in vitro research applications investigating growth hormone regulation mechanisms and related cellular pathways. The reconstituted peptide serves as a valuable tool for studying albumin-binding kinetics, proteolytic stability, and receptor binding affinity in controlled laboratory settings. Researchers should validate peptide activity through appropriate bioassays and analytical methods specific to their experimental objectives.
All content is intended for in vitro laboratory research purposes only. Not for human or animal consumption. Not intended to diagnose, treat, cure, or prevent any condition.
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All products on this site are for Research, Development use only. Products are Not for Human consumption of any kind. The statements made within this website have not been evaluated by the US Food and Drug Administration. The statements and the products of this company are not intended to diagnose, treat, cure or prevent any disease.
ElementSarms is a chemical supplier. ElementSarms is not a compounding pharmacy or chemical compounding facility as defined under 503A of the Federal Food, Drug, and Cosmetic act. ElementSarms is not an outsourcing facility as defined under 503B of the Federal Food, Drug, and Cosmetic act.
Sarms
Stacks
Research Liquids
Albuterol 5MG/ML | 30ML with dropper
Anastrozole 1.5MG/ML | 30ML with dropper
Clomiphene 50MG/ML | 30ML with dropper
Finasteride 5MG/ML | 30ML with dropper
Letrozole 3.5 MG/ML | 30ML with dropper
LiquiCia 30MG/ML | 30ML with dropper
LiquiCia T50 50MG/ML | 30ML with dropper
LiquiClen 200MCG/ML | 30ML with dropper
Liquistane / Exemestane 25MG/ML | 30ML with dropper
LiquiTamo 20MG/ML | 30ML with dropper
LiquiVia 25MG/ML | 30 ML with dropper
T3 LIOTHYRONINE 200MCG/ML | 30ML with dropper
Toremifene Citrate 60MG/ML | 30ML with dropper
Yohimbine HCL 10MG/ML | 30ML with dropper
Research Peptides
Aicar 50MG
BPC-157 + TB-500 Blend 2mg ea/ 4MG
BPC-157 5MG
CJC-1295 + DAC 2MG
CJC-1295 | No DAC 2MG
Epithalon 10MG
Frag Premium 176-191 5MG
GHK-CU Copper Peptide 50MG
GHRP-2 5MG
GHRP-6 5MG
Hexarelin 5MG
IGF-1 DES 1MG
IGF-1 LR3 1MG
Ipamorelin 5MG
Melanotan 2 10MG
NAD+ 500MG
PT-141 / Bremelanotide 10MG
GLP-1/GIP/GCG (RT)
Selank 5MG
GLP1 (SM)
Sermorelin 5MG
TB-500 5MG
GIP/GLP-1 (TZ)
PDE5 Inhibitors
GLP-1
Diluents
Bacteriostatic Water 10ML