Sensitivity 46.88 pg/mL Detection Range 78.13-5000 pg/mL Sample Volume 100 μL Total Assay Time 3 h 30 min Reactivity Human Specificity This kit recognizes Human GH in samples. No significant cross-reactivity or interference between Human GH and analogues was observed Recovery 80%-120% Sample Type Serum, plasma and other biological fluids Detection Method Colorimetric method, ELISA, Sandwich Assay Type Sandwich-ELISA Size 96T / 48T / 24T / 96T*5 / 96T*10 Storage 2-8°C Expiration Date 12 months
Typical data
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
pg/mL OD1 OD2 Mean OD Corrected OD 5000 2.299 2.397 2.348 2.256 2500 1.664 1.696 1.680 1.588 1250 0.895 0.867 0.881 0.789 625 0.586 0.570 0.578 0.486 312.5 0.293 0.309 0.301 0.209 156.25 0.195 0.189 0.192 0.100 78.13 0.144 0.144 0.144 0.052 0 0.092 0.092 0.092 -
Precision
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
/ Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 Numbers 20 20 20 20 20 20 Mean(pg/mL) 246.720 774.540 2266.960 240.190 804.030 2127.210 Standard deviation 13.740 33.770 153.930 19.380 61.750 169.750 CV(%) 5.570 4.360 6.790 8.070 7.680 7.980 Recovery
The recovery of Human GH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices. The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
Sample Type Range (%) Average Recovery(%) Cell culture media (n=8) 88-99 94 Serum (n=8) 92-105 99 EDTA plasma (n=8) 94-106 100 Linearity
Linearity of the assay was evaluated by spiking samples with high concentrations of Human GH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.
/ / Cell culture media (n=5) EDTA plasma (n=5) Serum (n=5) 1:2 Range 97-113 97-113 87-99 Average 105 104 94 1:4 Range 94-109 85-98 99-114 Average 100 92 106 1:8 Range 95-106 79-92 101-118 Average 100 86 107
Stability
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
/ Variation range of 37°C mean concentration / 2-8°C mean Sample 1(n=16) 89.07-98.48 Sample 2(n=16) 94.68-110.55
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GH. You can calculate the concentration of Human GH in the samples by comparing the OD of the samples to the standard curve.
IF:{{item.impact}}
Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}}
Reactivity:{{item.species}}
Sample Type:{{item.sample_type}}
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
Title Status Valid until Action {{v.文件名}} {{v.status_str}} {{v.timestamp_str}} Download /
*Note:Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.