TB-500 Dosing Protocol: 5 mg Vial — Loading & Maintenance Schedule
Thymosin Beta-4 (TB-500) dosing guide for the 5 mg vial — loading and maintenance phases for systemic tissue repair and anti-inflammatory effects.
Quickstart highlights
TB-500 is a synthetic peptide corresponding to the active fragment (Ac-SDKP, 17 amino acids from the C-terminus) of Thymosin Beta-4 — a 43-amino acid actin-sequestering protein ubiquitous in human tissue.
Concentration: 2 mg/mL (5 mg vial + 2.5 mL bacteriostatic water).
At 2 mg/mL: 2 mg = 100 units on a U-100 syringe.
Loading phase: 2 mg 2x weekly for 4–6 weeks; maintenance: 2 mg every 2 weeks.
TB-500 acts systemically — inject in abdomen, not at injury site.
One 5 mg vial provides ~2.5 loading doses (2 mg each); plan 4 vials for a 6-week loading phase.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
1-6
2000
100
Twice weekly
2 mg 2x weekly — 100 units each; loading phase; one 5 mg vial provides ~2.5 injections
7+
Every 2 weeks
2 mg every 2 weeks — maintenance phase; one 5 mg vial provides 2 maintenance doses
Gradual approach
1-8
1000
50
1 mg 2x weekly — conservative loading; suitable for sensitive patients or first TB-500 cycle
9-12
Advance to 2 mg 2x weekly if 1 mg well-tolerated
Your free TB-500 tracker
Dose reminders
Injection logging
Cycle progress
Reconstitution steps
Draw 2.5 mL bacteriostatic water; inject slowly down the vial wall.
Swirl gently until lyophilized powder dissolves; do not shake — TB-500 is susceptible to degradation from mechanical agitation.
Final concentration: 2 mg/mL. At 2 mg/mL: 2 mg = 100 units on a U-100 syringe.
Label with date; refrigerate at 2–8 °C. Use within 28 days.
Supplies needed
8-week plan
4 vials
16 syringes
10 mL bac water
16 alcohol swabs
12-week plan
7 vials
24 syringes
18 mL bac water
24 alcohol swabs
Protocol overview & cycle notes
Promote systemic tissue repair, reduce inflammation, and accelerate injury recovery through the TB-500 loading-then-maintenance protocol, leveraging Thymosin Beta-4's unique actin-sequestering and stem cell-mobilizing properties.
Cycle length: 12 weeks on.
Off-cycle: 4–8 weeks off after 12-week cycle.
Storage & handling
Injection & tracking tips
TB-500 has systemic effects unlike BPC-157 — injection site does not need to be near the injury; abdomen is preferred for reliable subcutaneous absorption.
Do not shake the reconstituted vial; TB-500 (as a fragment of the 43-amino acid Thymosin Beta-4) is more prone to mechanical degradation than smaller peptides.
For combination with BPC-157: use separate syringes and separate injection sites; TB-500 and BPC-157 can be used on the same days.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
Track injury pain (0–10 VAS) and range of motion weekly during loading phase.
Log systemic effects: fatigue levels, exercise recovery, and any soft tissue inflammation indicators.
Measure inflammatory markers (CRP, ESR) at baseline and after 6 weeks if clinically indicated.
How this works & references
Frequently asked questions
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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.
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