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RayBio® Human GDF-8/Myostatin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
As low as $449.00
Product Description
Specifications
Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit Estimated Lead Time 1-2 business days Species Human Sensitivity 0.83 ng/ml
Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by SimoaTM technology. Detection Range 0.83 ng/ml - 200 ng/ml Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin-1 (CT-1), Cathepsin V, FABP1 (L-FABP), FGF-20, HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2 (Noelin-2), p53, PDECGF (Thymidine Phosphorylase), PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TPP1 (Tripeptidyl-peptidase I), TREM-2, TrkC, TROY (TNFRSF19), Uromodulin, XIAP. Compatible Sample Types Cell Culture Supernatants, Plasma, Serum Recommended Dilution (Serum/Plasma) 2 fold Accession Number O14793 Gene Id 2660 Gene Symbols MSTN GDF8 Protein Name / Synonyms Growth/differentiation factor 8Â (GDF-8) (Myostatin) Quantitative/Semi-Quantitative Quantitative Solid Support 96-well Microplate Design Principle Sandwich-based Method Of Detection Colorimetric Shipping Type Blue ice Storage -20°C
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Product Features
Additional Notes
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required
Distilled or deionized water Precision pipettes to deliver 2 μl to 1 μl volumes Adjustable 1-25 μl pipettes for reagent preparation 100 μl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 μl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Citations
Conditioned Media from Head and Neck Cancer Cell Lines and Serum Samples from Head and Neck Cancer Patients Drive Catabolic Pathways in Cultured Muscle Cells
Species:
Human
Sample Type:
Plasma
Year:
2023
Journal Name:
Cancers
Resistance Exercise-Induced Increases in Muscle Myostatin mRNA and Protein Expression Are Subsequently Decreased in Circulation in the Presence of Increased Levels of the Extracellular Matrix Stabilizing Protein Decorin
Species:
Human
Sample Type:
Serum
Year:
2022
Journal Name:
Journal of Sports Science and Medicine
Larrosa, Food Funct., 2020, DOI: 10.1039/D0FO00246A.
Species:
Human
Sample Type:
Plasma
Year:
2020
Increasing lean muscle mass in mice via nanoparticle-mediated hepatic delivery of follistatin mRNA
Species:
Mouse
Sample Type:
Serum (Nanoparticle testing)
Year:
2018
Journal Name:
Theranostics
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