TB-500 (Thymosin Beta-4) is one of the most effective peptides for healing injuries, but dosing is confusing. Too little won't deliver results; too much wastes expensive product. This evidence-based TB-500 dosage chart shows exactly how much to use for tendonitis, muscle tears, joint injuries, and recovery.
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TB-500 Dosage Chart: Quick Reference
LOADING PHASE (Weeks 1-4): • Injury Healing: 5-10mg per week (split into 2-3 doses) • Severe injury (torn tendon/muscle): 10mg per week • Mild injury (tendonitis, strain): 5-7.5mg per week • Frequency: 2-3 injections per week (e.g., Monday/Thursday or Mon/Wed/Fri)
MAINTENANCE PHASE (Weeks 5+): • Dosage: 2.5-5mg per week (single dose or split) • Frequency: Once or twice per week • Duration: 4-8 weeks total, then reassess
PREVENTION/RECOVERY: • Dosage: 2.5mg per week • Frequency: Once weekly • Use case: Athletes preventing injury or speeding general recovery
TB-500 Dosage by Injury Type
Different injuries respond to different dosing strategies:
Tendonitis (Tennis Elbow, Achilles, Rotator Cuff)
• Loading: 5-7.5mg/week for 4 weeks • Maintenance: 2.5-5mg/week for 4-6 weeks • Injection site: Subcutaneous (abdomen) or near injury • Expected results: Noticeable improvement in 2-3 weeks
Muscle Tears or Strains
• Loading: 7.5-10mg/week for 4 weeks • Maintenance: 5mg/week for 4 weeks • Injection site: Subcutaneous, close to injury if possible • Expected results: Faster recovery, reduced scar tissue
Joint Injuries (Knee, Shoulder, Hip)
• Loading: 5-10mg/week for 4-6 weeks • Maintenance: 2.5-5mg/week for 4-8 weeks • Stack with: BPC-157 for localized healing • Expected results: Reduced inflammation, improved mobility
Post-Surgery Recovery
• Dosage: 5mg/week starting 1 week post-op • Duration: 6-8 weeks • Note: Consult surgeon before use; TB-500 accelerates tissue repair
How to Inject TB-500: Step-by-Step
Reconstitution: 1. TB-500 typically comes in 5mg vials 2. Add 2ml bacteriostatic water for easy dosing (1ml = 2.5mg) 3. Inject water slowly down the vial side, don't shake 4. Gently swirl until fully dissolved 5. Store in refrigerator (good for 30 days)
Injection: 1. Draw desired dose (e.g., 0.5ml for 1.25mg, 1ml for 2.5mg) 2. Subcutaneous injection (pinch fat, inject at 45° angle) 3. Preferred sites: abdomen, thigh, or near injury 4. Rotate injection sites to prevent scar tissue 5. Insulin syringes (0.5ml or 1ml, 29-31 gauge) work best
TB-500 Injection Frequency: Does Timing Matter?
TB-500 has a long half-life (~7-10 days), so precise timing isn't critical, but consistency matters:
• 2x per week: Monday/Thursday or Tuesday/Friday (most common) • 3x per week: Mon/Wed/Fri (higher total dose weeks) • 1x per week: Maintenance phase only
Does injection timing affect results? No strong evidence suggests morning vs. evening makes a difference. Some users inject post-workout for perceived synergy, but this isn't scientifically proven. Consistency is more important than timing.
TB-500 Dosage for Athletes vs. Injury Recovery
Injury Recovery (Priority: Healing): • Aggressive loading phase: 10mg/week • Targeted to specific injury • Short-term use (8-12 weeks)
Athletic Performance/Prevention (Priority: Recovery): • Conservative dosing: 2.5-5mg/week • General systemic benefits • Can cycle on/off throughout season • Helps prevent overuse injuries
Bodybuilding (Rare Use Case): TB-500 isn't primarily a muscle-building peptide. It's for injury prevention during heavy training. If this is your goal, 2.5mg/week year-round is more cost-effective than higher doses.
Common TB-500 Dosing Mistakes
1. Skipping the Loading Phase Many users start at 2.5mg/week and wonder why it's not working. The loading phase (5-10mg/week) builds up serum levels necessary for healing. Don't skip it.
2. Stopping Too Soon TB-500 works slowly. Many injuries need 6-8 weeks minimum. Stopping at 4 weeks because you 'feel better' often leads to relapse.
3. Using Expired or Improperly Stored Product Reconstituted TB-500 lasts ~30 days refrigerated. After that, potency drops. Don't reconstitute entire supply at once unless you'll use it within a month.
4. Injecting Only at Injury Site TB-500 works systemically. It doesn't need to be injected directly into the injury. Subcutaneous (belly fat) is just as effective and less painful.
5. Not Combining with BPC-157 For serious injuries, TB-500 (systemic healing) + BPC-157 (localized healing) is the gold standard stack. Using one without the other leaves results on the table.
TB-500 + BPC-157 Stack Dosing
The most popular peptide stack for injury healing:
Loading Phase (4 weeks): • TB-500: 5-10mg/week (2-3x weekly) • BPC-157: 250-500mcg/day (daily injections)
Maintenance Phase (4-6 weeks): • TB-500: 2.5-5mg/week (1-2x weekly) • BPC-157: 250mcg/day or stop
Why stack them? • TB-500 = systemic healing (tendons, ligaments, muscles) • BPC-157 = localized healing (accelerates repair at injury site) • Together = faster recovery + less scar tissue
For collagen remodeling support during healing, consider adding GHK-Cu to the stack.
Frequently Asked Questions
For injury healing: 5-10mg/week during loading phase (weeks 1-4), then 2.5-5mg/week maintenance. For general recovery: 2.5mg/week is sufficient.
You can, but it's unnecessary and wasteful. TB-500 has a long half-life, so 2-3x per week is optimal. Daily injections don't improve results and cost significantly more.
Subcutaneous injection in the abdomen is easiest and most common. You can inject near the injury site, but systemic (belly/thigh) injections work just as well since TB-500 circulates throughout the body.
Most users notice improvement in 2-4 weeks with proper loading dosing (5-10mg/week). Severe injuries may take 6-8 weeks. If you see no improvement after 6 weeks, dosing may be too low or the injury may require medical intervention.
For maintenance or prevention, yes. For active injury healing, 2.5mg/week is too low. You need 5-10mg/week during the loading phase to build sufficient serum levels.
TB-500 is typically used for 8-12 week cycles, then discontinued once the injury heals. Long-term daily use isn't well-studied and is unnecessarily expensive. Some athletes use 2.5mg/week year-round for injury prevention.
The Bottom Line
TB-500 dosing follows a loading/maintenance approach: 5-10mg/week for 4 weeks, then 2.5-5mg/week. Clinical data and anecdotal success support this protocol.
Don't under-dose during the critical first month, and give the peptide 6-8 weeks minimum. For serious injuries, stack with BPC-157 for maximum healing. Source pharmaceutical-grade TB-500 and store reconstituted vials properly.
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