Product Description Recombinant Human Latent GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375.
Product Background Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Product Category Recombinant Protein / Antibody discovery / Antibody discovery | Others
Human Latent GDF-8 Protein (pro&latent) (GDF-HM108) Specifications/Details
Protein GDF-8
Synonyms Myostatin; GDF8; Latent GDF8; Mstn
Accession
O14793
Species Human
Biotinylated no
Amino Acid Range Asn24-Ser375
Molecular Weight The protein has a predicted MW of 41.19 kDa. Due to glycosylation, the protein migrates to 14 kDa, 38-40 kDa and 45-52 kDa based on Bis-Tris PAGE result.
Activity Immobilized Human Latent GDF-8, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Apitegromab, hFc Tag with the EC50 of 21.5 ng/ml determined by ELISA.
Product Tag N-His
Fluorophore Unconjugated
Expression System HEK293
Purity > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin Less than 1 EU per ug by the LAL method.
Shipping, Reconstitution, & Storage
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage -20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Other Available Proteins:
Finding available proteins...
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