TB-500 (10 mg Vial) Dosage Protocol
TB-500 Dosage Chart
TB-500 is dosed at 500 mcg–1 mg daily via subcutaneous injection in educational protocols. A 10 mg vial reconstituted with bacteriostatic water yields about 3.33 mg/mL. This information is for research and educational use only.
Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.
Typical daily range: 500–1000 mcg once daily (gradual titration recommended).
Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‐100 insulin syringe.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); do not freeze reconstituted solution.
TB‐500 is a synthetic peptide fragment corresponding to the active region of thymosin beta‐4 (Tβ4), a naturally occurring 43‐amino‐acid protein involved in tissue repair and regeneration[1][2]. This educational protocol presents a once‐daily subcutaneous approach using a practical dilution for accurate insulin‐syringe measurements in research settings.
Research context: For evidence on mechanisms, human and preclinical research, limitations, and safety, read TB-500 Peptide: Benefits, Uses, Side Effects, Dosage, and Research.
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
Weeks 1–2
500 mcg
15 units (0.15 mL)
Weeks 3–4
600 mcg
18 units (0.18 mL)
Weeks 5–8
750 mcg
23 units (0.23 mL)
Weeks 9–12
1000 mcg
30 units (0.30 mL)
Frequency: Inject once daily subcutaneously. This schedule uses the largest practical dilution (3.0 mL) to keep per‐injection units in a comfortable range for accurate measurement. Total weekly dose averages ~5 mg, consistent with research protocols[3][4].
Reconstitution Steps
Draw 3.0 mL bacteriostatic water with a sterile syringe.
Inject slowly down the vial wall; avoid foaming.
Gently swirl/roll until dissolved (do not shake).
Label with date and concentration; refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Supplies Needed
Plan based on an 8–16 week daily protocol with gradual titration.
Peptide Vials (TB‐500, 10 mg each):
8 weeks ≈ 4 vials
12 weeks ≈ 7 vials
16 weeks ≈ 10 vials
Insulin Syringes (U‐100):
Per week: 7 syringes (1/day)
8 weeks: 56 syringes
12 weeks: 84 syringes
16 weeks: 112 syringes
Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
8 weeks (4 vials): 12 mL → 2 × 10 mL bottles
12 weeks (7 vials): 21 mL → 3 × 10 mL bottles
16 weeks (10 vials): 30 mL → 3 × 10 mL bottles
Alcohol Swabs: One for the vial stopper + one for the injection site each day.
Per week: 14 swabs (2/day)
8 weeks: 112 swabs → recommend 2 × 100‐count boxes
12 weeks: 168 swabs → recommend 2 × 100‐count boxes
16 weeks: 224 swabs → recommend 3 × 100‐count boxes