製品説明
TB500 is a synthetic version of an active region of thymosin β4. TB500 exhibits anti-fibrotic and wound healing activities by inhibiting the Akt signaling pathway and binding to actin. TB500 is claimed to promote endothelial cell differentiation, angiogenesis in dermal tissues, keratinocyte migration, collagen deposition and decrease inflammation[1][2][3]. 体外実験
TB500 exhibits tissue repairing and regenerative activities through its active ingredient Ac-Tβ417-23. Ac-Tβ417-23 prevents apoptosis, promotes cell survival and angiogenesis, stimulates T cell proliferation, exhibiting immunomodulatory activity[2]. TB500 (15-25 μg/mL, 2 days) promotes cell migration and wound healing by enhancing ATP-induced increase in intracellular calcium ions and activating purinergic P2 receptors[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Migration Assay [3]
Cell Line: PAM212 Concentration: 15-25 μg/mL Incubation Time: 2 days Result: Promoted cell migration. 体内実験
TB500 (10 mg/dose, single dose) can be used as doping agent in horse and greyhound racing[2]. TB500 (15 μg/30 μL, topical administration, once daily for 10 days) promotes wound healing, and exhibits good tissue repair effects in BALB/c mouse models[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model: BALB/c mouse skin wound models[3] Dosage: 15 μg/30 μL Administration: 15 μg/30 μL, topical administration, once daily for 10 days Result: Accelerated wound healing. 臨床実験
分子量
889.01 分子式
C38H68N10O14 CAS 番号
885340-08-9 Appearance
Solid Color
White to off-white Sequence
N-Acetyl-Leu-Lys-Lys-Thr-Glu-Thr-Gln Sequence Shortening
Ac-LKKTETQ 輸送条件
Room temperature in continental US; may vary elsewhere. 保管条件
polypeptide, sealed storage, away from moisture and light, under nitrogen 溶剤 & 溶解度
体外:
H2O : ≥ 200 mg/mL (224.97 mM)
*"≥" means soluble, but saturation unknown.
Preparing Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1248 mL 5.6242 mL 11.2485 mL 5 mM 0.2250 mL 1.1248 mL 2.2497 mL 10 mM 0.1125 mL 0.5624 mL 1.1248 mL
View the Complete Stock Solution Preparation Table
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Molarity Calculator
Dilution Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
一般には略語で表示されます:C1V1 = C2V2 濃度 (開始)
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C2
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V2
In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). 純度とドキュメンテーション
純度: 99.57%
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COA (249 KB) RP-HPLC (266 KB) LCMS (95 KB) Elemental Analysis Report (108 KB)
取扱説明書 (2659 KB) 参考文献
[1]. Emmie N M Ho, et al. Doping control analysis of TB-500, a synthetic version of an active region of thymosin β4, in equine urine and plasma by liquid chromatography-mass spectrometry. J Chromatogr A. 2012 Nov 23;1265:57-69. [Content Brief]
[2]. Esposito S, et al., Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential. Drug Test Anal. 2012 Sep;4(9):733-8. [Content Brief]
[3]. Huang CM, et al., In vivo detection of secreted proteins from wounded skin using capillary ultrafiltration probes and mass spectrometric proteomics. Proteomics. 2006 Nov;6(21):5805-14. [Content Brief]
[4]. Shah R, et al., Thymosin β4 inhibits PDGF-BB induced activation, proliferation, and migration of human hepatic stellate cells via its actin-binding domain. Expert Opin Biol Ther. 2018 Jul;18(sup1):177-184. [Content Brief]