TB-500 (Ac-LKKTETQ) Dosage Guide, Benefits & Side Effects
This active Thymosin Beta-4 fragment sequesters actin to speed tendon, cardiac, and neurological recovery from a single injection site.
Chemical Makeup
7
Amino Acids
TB-500 (Ac-LKKTETQ)
Leu
Position 1
Lys
Position 2
Position 3
Thr
Position 4
Glu
Position 5
Position 6
Gln
Position 7
As seen on
TB-500 (Ac-LKKTETQ) Dosage Guide for Recovery & RepairTB-500 (Ac-LKKTETQ) Dosage Calculator
This is a smaller fragment version of TB-500 that still helps regenerate tissue and reduce inflammation.
Vial size
10 mg
Bacteriostatic water
3 mL
Dosing
1–2 mg
Frequency
Every 2 days
Cycle
4–6 weeks
Benefit
Recovery & Repair
What is TB-500 (Ac-LKKTETQ)?
TB-500 is a lab-made peptide based on a small fragment of a natural repair protein found throughout the body (Thymosin Beta-4). It works by helping cells move to where they're needed and supporting new blood vessel growth, both of which speed up healing. It's used mainly in research and by athletes seeking faster recovery from injuries to muscles, tendons, and ligaments.
Healing
Research targets include tissue repair, wound healing, and recovery from injury.
Longevity
Research targets include cellular aging, senescence, and long-term healthspan.
tb50
Is TB-500 (Ac-LKKTETQ) FDA approved?
TB-500 (Ac-LKKTETQ) is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.
Research Use Only
TB-500 (Ac-LKKTETQ) has not been evaluated by the FDA for safety or efficacy in humans.
No Clinical Oversight
Not manufactured under FDA-regulated quality or clinical standards.
Unregulated Sourcing
Purity, dosing, and sourcing are not verified through FDA testing or oversight.
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How Does TB-500 (Ac-LKKTETQ) Work?
It grabs onto a building-block protein inside cells called actin, helping control how quickly a cell can reorganize its internal scaffolding to move. This is what allows it to help cells migrate rapidly to an injury site, support new blood vessel growth, and calm inflammation there.
Grabs onto actin
Attaches to a building-block protein inside cells called actin.
Reorganizes the cell scaffold
Controls how quickly cells can rebuild their internal structure to move.
Speeds healing
Helps cells migrate to the injury, supports vessel growth, and calms inflammation.
TB-500 (Ac-LKKTETQ) Targeted areas
How to reconstitute TB-500 (Ac-LKKTETQ)
The materials you'll need and step-by-step instructions for safely mixing TB-500 (Ac-LKKTETQ) with bacteriostatic water.
Materials needed
Your TB-500 (Ac-LKKTETQ) vial (lyophilized)
Alcohol swabs
Bacteriostatic sterile water
3 mL syringes (Luer Lock tip)
25G or 27G needles (Luer Lock). Other gauges may also be acceptable.
Sharps container (optional)
Remove the caps
Sanitize the rubber stoppers
Attach the needle
Draw the bac water
Pull back on the plunger to draw your desired volume of bacteriostatic water. If you overfill, just push the excess back in until you reach the right marker on the syringe.
Insert the needle into the TB-500 (Ac-LKKTETQ) vial
With the bac water in your syringe, insert the needle into the TB-500 (Ac-LKKTETQ) vial at a slight angle to avoid pressure buildup.
Release the water gently
Let the water run gently down the side of the vial. Don't inject it forcefully.
Swirl to dissolve
Avoid shaking. Gently swirl, flip, and roll the vial to dissolve the powder.
Check for full dissolution
Cap, dispose, and store
How to Store TB-500 (Ac-LKKTETQ)
Freeze the dry powder for long-term storage, or refrigerate it for shorter periods, protected from light and moisture. Once mixed with liquid, refrigerate and use within about a month, and don't freeze it once mixed.
Lyophilized Storage
-20°C long-term or 2–8°C short-term, protected from light and moisture.
Reconstituted Storage
Refrigerate at 2–8°C, use within 28 days.
Handling Notes
Do not freeze the reconstituted solution.
What Are the Benefits of TB-500 (Ac-LKKTETQ)?
What research says it may help with, and how it works in the body.
tissue repair
Its active core piece helps skin wounds heal.
In research, it supports tissue repair about as well as the full-length parent protein, thymosin beta-4.
It helps skin cells move into a wound to close it and encourages the skin to lay down new collagen, the protein that gives skin its structure.
antiinflammatory
It reduces inflammation in tissue that has been damaged.
vascular support
It helps the cells that line blood vessels mature properly and encourages the growth of new blood vessels.
What Are the Side Effects of TB-500 (Ac-LKKTETQ)?
Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.
Who Should Avoid It
Not authorized for medicinal use
Active cancer (theoretical concern)
Pregnancy or breastfeeding
Prohibited by WADA in sports
Stop Right Away If You Notice
Allergic reactions
Unusual swelling or inflammation
Milder Signs to Watch For
How Long Should a TB-500 (Ac-LKKTETQ) Cycle Last?
This breaks down how long a typical TB-500 (Ac-LKKTETQ) cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.
That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.
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Cheat Sheets Featuring TB-500 (Ac-LKKTETQ)
See how TB-500 (Ac-LKKTETQ) fits into a broader research stack with these free downloadable cheat sheets. Each one includes reference dose ranges, dosing frequency, and half-life for every peptide it covers. For research purposes only.
18 peptides
42 peptides
78 peptides
TB-500 (Ac-LKKTETQ) Research References
It is a preclinical compound
TB-500 (Ac-LKKTETQ) is a preclinical compound
TB-500 contains Ac-LKKTETQ, the active site responsible for actin binding and wound healing.
n.d.
TB-500 is a synthetic version of the active region of thymosin beta-4.
TB-500 undergoes serial cleavage at C-terminus; acetylation protects N-terminus.
Characterized human metabolism of TB-500 using liver microsomes and S9 fraction.
Frequently Asked Questions About TB-500 (Ac-LKKTETQ)
Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.
What is TB-500 (Ac-LKKTETQ)?
TB-500 is the common name for a synthetic, N-acetylated 7-amino-acid peptide (Ac-LKKTETQ) that corresponds to the 'actin-binding domain' fragment (amino acids 17-23) of thymosin beta-4, a naturally occurring protein involved in cell migration, wound healing, and blood vessel formation. The acetyl group on one end is intended to slow enzymatic breakdown compared to the unmodified fragment. It is sold and studied strictly as a research chemical, not as an approved drug or supplement.
How is TB-500 typically dosed in research protocols?
Published research and vendor protocols commonly describe a 'loading phase' of roughly 4-6 mg total per week (often split into two subcutaneous doses of 2-2.5 mg, injected 2-3 days apart) for the first 4-6 weeks, followed by a lower maintenance dose of about 2 mg once weekly or every other week if continued. These figures come from research-use guidance and anecdotal protocols rather than clinical dosing standards, since there is no FDA-approved dose for humans.
How long does TB-500 stay active in the body?
Reported plasma half-life after subcutaneous administration is roughly 7-10 days, with peak plasma levels appearing 2-4 hours after injection. Because of this long half-life, once- or twice-weekly dosing is generally described as sufficient to maintain steady-state levels, unlike faster-clearing peptides that require daily dosing.
What is TB-500 commonly stacked with, and why?
TB-500 is most frequently discussed alongside BPC-157 in what is often called the 'Wolverine stack.' The rationale given is that BPC-157 acts more locally at an injury site to drive angiogenesis and fibroblast activity, while TB-500 is described as promoting systemic cell migration, reduced inflammation, and broader tissue remodeling. A commonly cited combined protocol is daily BPC-157 (250-500 mcg) with TB-500 dosed once or twice weekly (2-5 mg) for several weeks, with the two peptides kept in separate vials/injections rather than mixed together.
What side effects have been reported with TB-500?
Commonly reported mild effects include fatigue, dizziness, headache, and redness or irritation at the injection site. Less common but more serious reports include fever, rash, hives, blistering at the injection site, muscle aches, or gastrointestinal upset. There are no controlled human clinical trials establishing a full safety profile, so both the short- and long-term risks remain poorly characterized; any adverse reaction is generally advised to prompt discontinuation and medical evaluation.
How should TB-500 be reconstituted and stored?
As sold, TB-500 is typically a lyophilized (freeze-dried) powder that must be reconstituted with bacteriostatic or sterile water before use. Vendor and research-community guidance commonly recommends storing the unreconstituted powder frozen or refrigerated, and once reconstituted, keeping the vial refrigerated (roughly 2-8°C) and using it within about 2-4 weeks, avoiding freeze-thaw cycles and vigorous shaking of the solution.
Is TB-500 legal to buy and use, and is it banned in sports?
TB-500 is not approved by the FDA (or equivalent regulators elsewhere) for human medical use and is sold in most markets labeled 'for research purposes only, not for human consumption.' It is also on the World Anti-Doping Agency (WADA) prohibited list, meaning it cannot be used by athletes subject to anti-doping testing. Buying, possessing, or using it as a human drug outside of a licensed research or clinical context may carry legal and regulatory risk depending on jurisdiction.
Does TB-500 actually work for injury healing?
Most of the evidence for TB-500's healing effects comes from preclinical (animal and cell culture) studies of thymosin beta-4 and its fragment, which suggest roles in cell migration, angiogenesis, and reduced scarring. Human evidence is largely anecdotal, drawn from user reports and unregulated clinical use rather than peer-reviewed controlled trials, so claims of efficacy in humans have not been rigorously established and should not be treated as proven medical benefits.