Protocol Overview
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 5mg/mL. Always use sterile techniques.
Materials Needed
10mg TB-500 vial (lyophilized powder)
2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
Sterile syringe and needle (18-22 gauge for drawing)
Alcohol swabs
Clean workspace
Step-by-Step Process
Preparation: Allow the TB-500 vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Reference · research convention, not a validated dose
TB-500 protocols typically have two phases: a loading phase (4–6 weeks) to build tissue levels, followed by a maintenance phase.
Loading (Weeks 1–6)
2x/week (Mon & Thu)
2.5mg (50 units)
5mg
Maintenance (Weeks 7+)
1x/week
2.5mg
Loading phase: 5mg/week • Maintenance: 2.5mg/week • Vial Duration: 1 week loading or 2 weeks maintenance (10mg vial)
Quick Dosing Reference (at 5mg/mL)
10
500
0.5mg
20
1,000
1mg
30
1,500
1.5mg
40
2,000
2mg
50
2,500
Administration Guidelines
Administer subcutaneously in the morning on an empty stomach
Rotate injection sites between abdomen, thigh, and upper arm
Store reconstituted peptide refrigerated at 36-46F (2-8C)
Use reconstituted solution within 30 days
Never freeze reconstituted peptides
Document administration times and observations
Storage Requirements
Lyophilized (powder)
Room temperature or refrigerated, protect from light
Reconstituted
Refrigerated 36-46F (2-8C), use within 30 days
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