Induced cytotoxicity of MPC-11 cells assay for Human Myostatin. Cell proliferation was measured to calculate the ED50, which is as expected less than 50 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Myostatin Propeptide has a predicted Mw of 27.8 kDa (but runs higher in the gel) and Myostatin has a pre
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Description
Myostatin, also known as GDF-8, a conserved member of the TGF-β superfamily, is an essential regulator of skeletal muscle mass and cardiac muscle development and function. Myostatin is a secreted protein that negatively regulates skeletal muscle growth by determining muscle fiber number and size. Myostatin binds one of the two activin type II receptors (ACTRIIA, ACTRIIB) to activate SMAD signaling. Myostatin also activates MAPK signaling through TAK1-MKK6 and Ras pathways. Inhibition of myostatin increases muscle mass in a number of human disease animal models, such as muscular dystrophy.
Activity Assay
Human Myostatin is fully biologically active when compared to standard. The activity is determined by induced cytotoxicity of MPC-11 cells and it is typically less than 50 ng/ml. This corresponds to an expected specific activity of 2.0 x 104 units/mg.
AA Sequence
DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS
Alternative Names
Fibroblast Growth Factor 8, FGF8, FGF 8, androgen-induced growth factor, AIGF, heparin-binding growth factor 8, HBGF-8
Citations Documents Contact Us
Product Details Length 109 / 218 aa Molecular Weight 12.8 / 24.8 kDa Structure Dimer Source E. coli Accession Number O14793(267-375) Purity ≥95% determined by reducing and non-reducing SDS-PAGE Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 5 mM sodium phosphate, 50 mM soldium chloride, pH 7.5 Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. Stability and Storage
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.