Price range: £70.00 through £210.00
Human insulin-like growth factor 1 (IGF-1) protein is used in the maintenance of human pluripotent stem cells (iPSC) and is necessary for cell growth in the absence of insulin. IGF-1 promotes the proliferation of many cell types, including embryonic stem cells (ESC) and mesenchymal stem cells (MSC).
Qkine IGF-1 is a highly pure and bioactive 8 kDa protein, Qkine growth factors are animal origin-free (AOF) and carrier-protein free (CF) for consistent stem cell culture.
This protein is also available as GMP compliant Cell Therapy Grade, to enquire email [email protected].
In stock
Orders are typically shipped same or next day (except Friday).
Price range: £70.00 through £210.00
1000 μg will be dispatched as 2 x 500 μg.
Buy online with secure credit card or purchase order. For any questions, please email [email protected]
Summary:
High purity human IGF-1 protein (Uniprot:P05019) 8 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA Resuspend in sterile-filtered water at >50 μg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Maintenance of pluripotency in iPSC
Cell culture media optimization and replacement of insulin in defined media
Alternative names
Species reactivity
IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM). Cells were treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
IGF-1 protein migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 μg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk047 batch #104331.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine IGF-1 remained bioactive in media for 7 days. Bioactivity was determined using an IGF-1-responsive firefly luciferase reporter assay. Transfected HEK293T cells were treated in triplicate for 4 hours with a serial dilution of IGF-1 which had been pre-incubated in conditioned media for 7 days. Firefly luciferase activity was measured and normalised to the control Renilla luciferase activity.
Product background
The insulin-like growth factors (IGFs) are a family of proteins with a similar structure to insulin. IGF-1 has an A and B chain connected by disulphide bonds, its similarity to insulin means it can bind with low affinity to the insulin receptor [1]. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic, and lung [1]. IGF-1 exerts its biological effect through binding to the IGF1 receptor (IGF1R), its effect is modulated through interaction with a group of binding proteins (IGFBPs), which tend to inhibit the effect of IGF-1 [2]. Through IGF1R the Ras-MAPK and PI3 kinase signaling pathways are activated leading to increased cell proliferation and survival [2].
IGF-1 can be used to support maintenance of human embryonic stem cell (ESC) [3] and mesenchymal stem cell (MSC) cultures [3, 4].
Background references
FAQ
IGF1R is linked to various cytoplasmic second messenger molecules including the RAS-MAPK and PI3K signaling networks.
IGF-1, also called somatomedin C, is a hormone with a similar molecular structure to insulin.
IGF-1 is primarily produced by the liver.
IGF-1 binds to the IGF-1 receptor (IGF1R)
IGF-1 can be used in embryonic stem cell (ESC) and mesenchymal stem cell (MSC) cultures to promote proliferation, as a replacement to insulin.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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