Sermorelin Dosing Protocol: 5 mg Vial — Daily Dosing Schedule & Supplies
Week-by-week subcutaneous dosing schedule for sermorelin GHRH analogue using the 5 mg vial, with reconstitution steps and supplies.
Quickstart highlights
Sermorelin acetate (GHRH 1-29 NH2) is a 29-amino acid analogue of endogenous growth hormone-releasing hormone (GHRH) that binds pituitary GHRH receptors with high affinity, stimulating pulsatile GH release through a physiological mechanism rather than direct GH administration.
Concentration: 2 mg/mL (5 mg vial + 2.5 mL bacteriostatic water).
At 2 mg/mL: 100 μg = 5 units, 200 μg = 10 units, 300 μg = 15 units, 500 μg = 25 units.
Inject nightly 30–60 minutes before sleep; avoid high-glycemic food 2 hours before.
One 5 mg vial provides 25 nightly doses at 200 μg or ~17 doses at 300 μg.
Combine with ipamorelin or GHRP-2 for synergistic GH secretion via complementary receptors.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
1-4
200
10
Once daily (before bed)
200 μg nightly — 10 units; one 5 mg vial provides 25 nightly doses
5-12
300
15
300 μg — 15 units; often combined with GHRP (ipamorelin/GHRP-2) for synergy
13+
500
25
500 μg maintenance — 25 units; maximum clinical dose in most protocols
Gradual approach
1-8
100
5
100 μg conservative start — ideal for sensitive individuals or first GH peptide cycle
9-16
Increase to 200 μg if 100 μg well-tolerated
Your free Sermorelin tracker
Dose reminders
Injection logging
Cycle progress
Reconstitution steps
Draw 2.5 mL bacteriostatic water into a 3 mL syringe; inject slowly down the vial wall.
Swirl gently until the lyophilized powder fully dissolves (1–2 minutes); do not shake.
Final concentration: 2 mg/mL (2,000 μg/mL). At this concentration, 1 unit on a U-100 syringe = 20 μg.
Label with date and concentration; refrigerate at 2–8 °C. Use within 20 days (based on 25 doses × 200 μg from 5 mg vial).
Supplies needed
8-week plan
1 vial
56 syringes
3 mL bac water
56 alcohol swabs
12-week plan
2 vials
84 syringes
5 mL bac water
84 alcohol swabs
Protocol overview & cycle notes
Restore physiological GH pulsatility through GHRH receptor stimulation with nightly sermorelin injections, targeting improved body composition, recovery, and sleep quality over a 12-week cycle.
Cycle length: 12 weeks on.
Off-cycle: 4–6 weeks off after every 12-week cycle; reassess IGF-1 levels before restarting.
Storage & handling
Injection & tracking tips
Inject subcutaneously 30–60 minutes before sleep — sermorelin acts on the hypothalamic-pituitary axis; endogenous GH pulsatility peaks during early sleep stages (NREM Stage 3).
Avoid food or high blood glucose for 2 hours before injecting; elevated insulin blunts GH secretion.
If combining with a GHRP (ipamorelin, GHRP-2), inject both at the same time from separate syringes at adjacent sites.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
Measure IGF-1 serum level at baseline, 6 weeks, and 12 weeks to quantify GH axis response.
Track sleep quality (subjective 0–10 and wearable deep sleep duration) — sermorelin typically improves delta-wave sleep within 4–6 weeks.
Record body composition changes (waist circumference, lean mass via InBody) every 8 weeks.
How this works & references
Sources
Source: Vittone J et al. — Sermorelin 500 μg nightly increases IGF-1 in GH-deficient adults. J Clin Endocrinol Metab. 1997
FDA — Geref (sermorelin acetate for injection) prescribing information
Polyethylene glycol-conjugated growth hormone-releasing hormone is long acting and stimulates GH in healthy young and elderly subjects. Munafo A et al. — Eur J Endocrinol — 2005
Frequently asked questions
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This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.
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