GLOW Blend Dosing Protocol — BPC-157, TB-500 & GHK-Cu Skin & Repair Guide
GLOW peptide blend (BPC-157 + TB-500 + GHK-Cu) dosing guide for tissue repair, angiogenesis, and collagen and connective-tissue remodeling.
Quickstart highlights
The GLOW blend combines BPC-157, TB-500, and GHK-Cu.
Three peptides: BPC-157 + TB-500 + GHK-Cu.
BPC-157: nitric oxide + VEGF angiogenesis for new capillary formation.
TB-500: actin-binding cell migration and wound closure.
GHK-Cu: collagen and elastin synthesis with matrix remodeling; a blue-green color is normal.
Evening injection can align with the overnight tissue-repair window.
Research-only: not FDA-approved; dosing is practitioner-reported, not from human trials.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
1-4
250
12.5
Once daily (evening)
250 μg of each component — 12.5 units; starting dose; 5+5 mg vial provides ~20 doses
5-12
500
25
Once daily
500 μg each — 25 units; therapeutic aesthetic dose; standard GLOW blend maintenance
Your free GLOW Blend tracker
Dose reminders
Injection logging
Cycle progress
Reconstitution steps
Reconstitute GLOW blend in 5 mL bacteriostatic water for approximately 2 mg/mL of each component.
Swirl gently; GHK-Cu produces the characteristic blue-green color.
Label with date; refrigerate at 2–8 °C. Use within 28 days.
The blue-green color from GHK-Cu copper chelation is normal and indicates active peptide.
Supplies needed
12-week plan
3 vials
84 syringes
15 mL bac water
84 alcohol swabs
Protocol overview & cycle notes
Support tissue repair and skin and connective-tissue rejuvenation through the GLOW blend's angiogenic (BPC-157), cell-migration (TB-500), and collagen and matrix-remodeling (GHK-Cu) mechanisms, administered via daily subcutaneous injection.
Cycle length: 12 weeks on.
Off-cycle: 8 weeks off; assess skin and hair quality changes photographically before resuming.
Storage & handling
Injection & tracking tips
Inject subcutaneously; evening dosing can align with the overnight tissue-repair window.
Reconstitute with bacteriostatic water and refrigerate; the blue-green tint from GHK-Cu is expected and indicates active copper chelation.
GLOW blend composition and ratios vary by supplier — confirm the three active peptides and concentrations before dosing.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
Standardized photographs at baseline, 4 weeks, and 12 weeks (same lighting, angle, camera).
Skin hydration measurement at baseline and 12 weeks if skin is the target.
Note injection-site tolerance and recovery or function changes weekly.
How this works & references
Sources
Source: Sikiric P et al. — BPC-157 cytoprotective and angiogenic effects. Curr Pharm Des. 2011. PMID: 21548867
Source: Goldstein AL, Hannappel E, Kleinman HK. — Thymosin beta4 and tissue repair. Trends Mol Med. 2005. PMID: 16099219
Source: Pickart L, Margolina A. — Regenerative actions of the GHK-Cu peptide. Int J Mol Sci. 2018. PMID: 29986520
Frequently asked questions
Related protocols
KLOW Blend Dosing Protocol — BPC-157, TB-500, KPV & GHK-Cu Guide
KLOW peptide blend (BPC-157 + TB-500 + KPV + GHK-Cu) dosing guide for tissue repair, anti-inflammatory support, and extracellular matrix remodeling — the GLOW blend plus KPV.
Vilon Dosing Protocol: 20 mg Vial — Dipeptide Immune & Longevity Bioregulator Guide
Vilon (Lys-Glu dipeptide) Russian immune and longevity bioregulator dosing guide — the simplest Khavinson bioregulator for systemic immune normalization and longevity support.
Vesugen Dosing Protocol: 20 mg Vial — Vascular Bioregulator & Cardiovascular Guide
Vesugen (Lys-Glu-Asp tetrapeptide) Russian vascular bioregulator dosing guide — endothelial cell gene normalization for arterial health, blood pressure regulation, and cardiovascular aging.
Testagen Dosing Protocol: 20 mg Vial — Testicular Bioregulator & Testosterone Support Guide
Testagen (Lys-Glu-Asp-Gly tetrapeptide) Russian testicular bioregulator dosing guide — Leydig cell gene normalization for testosterone production optimization and male reproductive aging support.
This content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.
Need help?