Figure 1. Dose-dependent accumulation of intracellular cAMP upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Gaylinn et al. (1993) Molecular cloning and expression of a human anterior pituitary receptor for growth hormone-releasing hormone. Molec. Endocr 7:77-84.
DeAlmeida and Mayo (1998) Identification and binding domains of the growth hormone-releasing hormone receptor by analysis of mutant and chimeric receptor proteins. Molec Endocr 12:750-765.