Recombinant human IGF-I (Insulin-like growth factor 1, somatomedin C), derived from the transfected human HEK293 cells.
As low as $135.00
Product Description
Specifications
Size 10 μg, 50 μg, 100 μg Estimated Lead Time 1-2 business days Species Human Accession Number P05019 Gene Id 3479 Gene Symbols IGF1 Protein Name / Synonyms Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C, Insulin-Like Growth Factor 1, IBP1, IGFI Expressed Region Gly49-Lys195 Expression System HEK293 cells Tag N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag Purity > 90% Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining Molecular Weight (kDa) Recombinant mouse IGF-I has a calculated molecular mass of 43 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 43-45 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. Format Lyophilized powder Formulation Lyophilized from a 0.2 μm filtered solution in PBS Concentration (lot specific) Determined by BCA protein assay kit (Thermo Scientific) Preservative None Reconstitution Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. Endotoxin Level Not determined Research Area Cancer, Inflammation Shipping Type Ambient temperature Storage -20°C
SDS-PAGE Image Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa). Lane 2: Untreated protein under reducing conditions. Lane 3: Treated protein with deglycosylation enzymes under native conditions. Lane 4: Treated protein with deglycosylation enzymes under reducing conditions. Lane 5: Deglycosylation mixture only without target proteins.
Activity
Stimulating cell growth significantly. Measured in a cell proliferation assay using MCF‐7 human breast cancer cells.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
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