Protocol Overview
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 2.5mg/mL. Always use sterile techniques.
Materials Needed
5mg Thymosin Beta-4 5mg vial (lyophilized powder)
2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
Sterile syringe and needle (18-22 gauge for drawing)
Alcohol swabs
Clean workspace
Step-by-Step Process
Preparation: Allow the Thymosin Beta-4 5mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Reference · research convention, not a validated dose
Monday
30
750mcg
Morning
Thursday
Weekly Total: 60 units (1,500mcg) • Vial Duration: ~17 days
Quick Dosing Reference · research convention, not a validated dose
4
100
0.1mg
10
250
0.25mg
20
500
0.5mg
750
0.75mg
40
1000
1mg
Administration Guidelines
Administer subcutaneously twice per week (e.g., Monday and Thursday)
Rotate injection sites between abdomen, thigh, and upper arm
Inject near area of injury when possible for localized research
Store reconstituted peptide refrigerated at 36-46F (2-8C)
Use reconstituted solution within 30 days
Never freeze reconstituted peptides
Document administration times, sites, and observations
Storage Requirements
Lyophilized (powder)
Room temperature or refrigerated, protect from light
Reconstituted
Refrigerated 36-46F (2-8C), use within 30 days
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